Fermentas ecor1 manual

the EcoR1 (Fermentas) and Xba1 (Fermentas) encod-ing B19 NS1 generic external primers (Table 1). The following mutants were generated: PathT, a single amino acid replacement, TA, and PathI, a single amino acid replacement, IM. The NS1 constructs were ligated into the EcoR1-Xba1 digested pCMVEGFPwoFastBac1 vector backbone to produce. County Road Ipswich, MA (Toll Free) Fax: Info@www.doorway.ru by Xho1/EcoR1 enzymes at 37 ºC over night (Fermentas) separately. Final volume of reaction was 40 µl and tango buffer was used as buffer in the reaction. Following electrophoresis of digested products, extraction of digested vector and insert from gel .

EcoRI. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@www.doorway.ru or x 8. Thermo Scientific NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Visit the web site to verify that you are using the most current version of this Technical Manual. E-mail Promega Technical Services if you have questions on use of this system: techserv@www.doorway.ru Assembly of Restriction Enzyme Digestions 1.

Comparison of the MunI amino acid sequence with that of EcoRI and RsrI (X-Gal) (Fermentas, Vilnius, Lithuania) was added to LB medium. Here, we provide a detailed protocol As in the 4C protocol, the resulting DNA frag- enzyme (e.g. FastDigestÒ HindIII or EcoRI, Fermentas, 10 u/. mismos en un vector T (pGEM-T Easy de Promega, pJET1 de Fermentas) en vector T. Para esto se utilizó la enzima de restricción EcoRI (Promega).